An experiment was conducted to quantify the anti-mullerian hormone (AMH) concentration in heifers and anestrus Murrah buffaloes during fortnight blood collections for 3 months. Two groups of heifer and anestrus Murrah buffaloes were formed on the basis of record and actual per rectal examination. Serum AMH concentration was determined by ELISA method. using bovine AMH ELISA kit. https://jalyttlers.shop/product-category/wax-discs/